If desired, subclone the DNA fragment encoding the GFP fusion protein into the pIRES vector. The bicistronic expression vector pIRES can be used to generate stable cell lines expressing GFP-tagged ...

This requires the ability to accurately track cells, observing their function, movement and interactions in vitro and in vivo. GFP ioMicroglia is built from the company’s well-established ioMicroglia.

Flow cytometric purification of GFP-expressing bone marrow cells. Twenty-four hours following the completion of the viral transduction procedure, bone marrow cells were depleted of RBCs and ...

Caption Primary HUVECS expressing GFP targeted to the mitochondria. One of the HUVEC is infected with Chlamydia, stained against HSP60 (magenta).These cells were treated with 1 um H2O2 which ...

ClonTech optimized GFP for expression in mammalian cells, licensed, and commercialized as enhanced GFP, or eGFP in 1996. While many groups worked with GFP to change its stability or activity, Roger ...