This was ingeniously exploited, and GFP was used as a reporter for the folding of upstream fusion proteins in E. coli. Our findings demonstrate that fusion of GFP to amino acidic tags also affects ...

they could amplify just the GFP gene by PCR, but that risked introducing mutations since PCR was error-prone at the time. Alternatively, they could transform the whole clone into E. coli, which would ...

Figure 1: GFP-fragment reassembly vectors and cloning linker sequences. To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives ...

The gene coding for GFP comes from the bioluminescent jellyfish ... Using a P-200 pipetman, add 100 microliters of competent E. coli to the "-DNA" tube. Place the tube back on ice. 7. Incubate both ...