This requires the ability to accurately track cells, observing their function, movement and interactions in vitro and in vivo. GFP ioMicroglia is built from the company’s well-established ioMicroglia.
After erylysis, cells labeled with Mac-1-Pe, B220-Pe, Gr-1-Pe and CD4-Pe (Pharmingen, Heidelberg, Germany), were analyzed for GFP-expression. Dead cells (propidium iodide-positive) were excluded.
If desired, subclone the DNA fragment encoding the GFP fusion protein into the pIRES vector. The bicistronic expression vector pIRES can be used to generate stable cell lines expressing GFP-tagged ...
ClonTech optimized GFP for expression in mammalian cells, licensed, and commercialized as enhanced GFP, or eGFP in 1996. While many groups worked with GFP to change its stability or activity, Roger ...
Mapping out gene expression is informative ... to other imaging-based techniques and successfully detected green fluorescent protein (GFP) mRNA in expected cell types and regions of the root tip of an ...
The axolotl (Ambystoma mexicanum) has extraordinary regenerative abilities—the salamander can regrow lost limbs and repair ...
Medical Xpress on MSN13d
The ‘Silent’ X Chromosome Gives the Aging Female Brain a BoostWhy do women's brains fare better in aging than men's? A study found that the second, 'silent' X chromosome turns on in the ...
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